CGEB - Integrated Microbiome Resource (IMR)

Submissions Guidelines

Contact the IMR Manager directly to initiate your project's consultation, then download our Excel form:

Sample Submission Form

This form is to be filled out with all of your details for tracking purposes and has sample preparation and shipping guidelines (please pay careful attention for international clients). You will be given a unique project/sample identifier in the form of "IMR-YYYYMM-00" which will allow sample tracking, will show up on your billing information and be listed in the online queue.

In order to offer as flexible (and economical) a service as possible to the community, we will not be insisting that clients fulfill complete multiplexed runs of samples. We offer the option of sequencing less samples, but with the caveat that your samples may take a bit longer in the pipeline as we wait for the remainder of the run slots to be filled by other projects/individuals. Of course, you will always have the option of expediting your service by purchasing the complete run at any time (even with less than the maximum of samples for that particular run type).

Sample Minimum Requirements

For amplicon sequencing (MiSeq):

Concentration: >1 ng/µL (preferrably >10 ng/µL); we do not re-quantify incoming DNAs for amplicon PCRs
Volume: 10 µL of each sample (enough for two different targets; ie: 16S + 18S)
Quality: "clean" DNA coming from commercial kit or proven manual method; absolutely clean spec ratios not required as PCR is relatively robust, but inhibitors must be removed

For client-prepared pool of amplicons (MiSeq):

purified final library at >10 ng/µL in at least 10 µL; we re-quantify incoming libraries (not oligos below) using Qubit
if using custom primers, provide 10 µL each of Read1 + Read2 + Index primers at 100 µM

For metagenomic sequencing (MiSeq/NextSeq):

Concentration: >0.2 ng/µL (preferrably >1 ng/µL); we re-quantify incoming DNAs using Qubit or Quant-iT
Volume: 10 µL of each sample
Quality: spec A260/280 and A260/230 ratios as close to 1.8 and 2.0 as possible (or over), since library prep (Nextera XT) is sensitive to DNA quality

For genome sequencing (MiSeq/NextSeq):

Concentration: >0.2 ng/µL (preferrably >1 ng/µL); we re-quantify incoming DNAs using Qubit or Quant-iT
Volume: 10 µL of each sample
Quality: spec A260/280 and A260/230 ratios as close to 1.8 and 2.0 as possible (or over), since library prep (Nextera XT) is sensitive to DNA quality